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Product Name
Anti-Nrf2 Rabbit antibody
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Description
Nrf2 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, ELISA
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: The antiserum was produced against synthesized peptide derived from human Nrf2. AA range:556-605
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Clonality
Polyclonal
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Formulation
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/100 - 1/300
IF: 1/200 - 1/1000
ELISA: 1/40000
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Validations
Immunofluorescence analysis of HUVEC cells, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Nrf2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HUVEC cells, using Nrf2 Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HuvEc cells using Nrf2 Polyclonal Antibody diluted at 1:1000
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Background
Swiss-Prot Acc.Q16236.Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress and the regulation of cellular redox conditions. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"