Anti-CSK (5E10) Mouse antibody
CSK (5E10) Mouse monoclonal antibody
WB, ICC/IF, FC
Human, Mouse, Monkey, Rat
Antigen: Purified recombinant fragment of human CSK expressed in E. Coli.
Ascitic fluid containing 0.03% sodium azide.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
Western blot analysis of extracts from Ramos,NTERA2,COS7,Jurkat and Hela cell lysates using Csk mouse mAb (1:1000 diluted). Predicted band size:50KDa. Observed band size:50KDa.
Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).
Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Swiss-Prot Acc.P41240.Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"