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Product Name
Anti-beta 2 Microglobulin (5G12) Mouse antibody
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Description
beta 2 Microglobulin (5G12) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human
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Isotype
IgG2a
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Preparation
Antigen: Purified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
Western blot detection of β2-microglobulin in Jurkat,A549,MCF7 and Hela cell lysates using β2-microglobulin mouse mAb (1:2000 diluted).Predicted band size:14KDa.Observed band size:14KDa.
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using B2M mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P61769.This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"