Beta-2-microglobulin antibody

Cat.#: 108486

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Product Information

  • Product Name
    Beta-2-microglobulin antibody
  • Documents
  • Description
    Beta-2-microglobulin Rabbit Polyclonal antibody. Positive IHC detected in human oesophagus cancer tissue, human stomach cancer tissue. Positive IF detected in A431 cells. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, human heart tissue, human stomach tissue, mouse lung tissue, Raji cells. Observed molecular weight by Western-blot: 12 kDa
  • Tested applications
    ELISA, WB, FC, IHC, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    B2M antibody; Beta-2-microglobulin antibody; HDCMA22P antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Beta-2-microglobulin recombinant protein (Accession Number: NM_004048). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117131(B2M antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117131(B2M antibody) at dilution of 1:1000

    1X10^6 HeLa cells were stained with 0.2ug B2M antibody (Catalog No:117131, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug B2M antibody (Catalog No:117131, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of (-20oc Ethanol) fixed A431 cells using Catalog No:117131(B2M Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed A431 cells using Catalog No:117131(B2M Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
  • References
    • Ma Y, Shen B, Zhang X. Heritable multiplex genetic engineering in rats using CRISPR/Cas9. PloS one. 9(3):e89413. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"