A primary antibody's Fc domain is constant within the same animal species, therefore, a given secondary antibody can be used with any primary antibody of the same isotype and target species. This reduces the cost by labeling only one type of secondary antibody. In addition, secondary antibodies offer increased sensitivity through the signal amplification.
At NovoPro, secondary antibodies are purified from the serum of several hosts, including goat, donkey, mouse, rabbit, chicken and sheep. Secondary antibodies are available with specificity for whole Ig molecules or antibody fragments such as the Fc or Fab regions. The conjugated secondary antibodies can be used for various immunology analysis and protein assays.