WTAP Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HEK-293 cells, L02 cells, MCF7 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 50-55kd
ELISA, IP, WB
Human,Mouse,Rat; other species not tested.
DKFZp686F20131 antibody; Female lethal(2)D homolog antibody; hFL(2)D antibody; KIAA0105 antibody; WT1 assOCiated protein antibody; WTAP antibody
This antibody was obtained by immunization of WTAP recombinant protein (Accession Number: BC000383). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116974(WTAP antibody) at dilution of 1:1000
IP Result of anti-WTAP (IP:Catalog No:116974, 3ug; Detection:Catalog No:116974 1:500) with HEK-293 cells lysate 2500ug.
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle.
- Moindrot B, Cerase A, Coker H. A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing. Cell reports. 12(4):562-72. 2015.
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