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Product Name
WTAP antibody
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Description
WTAP Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human testis tissue, Jurkat cells, mouse testis tissue. Positive IF detected in A431 cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp686F20131 antibody; Female lethal(2)D homolog antibody; hFL(2)D antibody; KIAA0105 antibody; WT1 assOCiated protein antibody; WTAP antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of WTAP recombinant protein (Accession Number: NM_152858). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107544(WTAP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:107544(WTAP antibody) at dilution of 1:50 (under 40x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107544(WTAP Antibody) at dilution of 1:2000
Immunofluorescent analysis of A431 cells using Catalog No:107544(WTAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle.
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References
- Liu J, Yue Y, Han D. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nature chemical biology. 10(2):93-5. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"