VPS35 Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in HepG2 cells, mouse brain tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 90-98kd
ELISA, WB, IF, IP
Human,Mouse,Rat; other species not tested.
DKFZp434E1211 antibody; DKFZp434P1672 antibody; FLJ10752 antibody; FLJ13588 antibody; FLJ20388 antibody; hVPS35 antibody; Maternal embryonic 3 antibody; MEM3 antibody; Vesicle protein sorting 35 antibody; VPS35 antibody
This antibody was obtained by immunization of VPS35 recombinant protein (Accession Number: BC002414). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116784(VPS35 Antibody) at dilution of 1:600
IP Result of anti-VPS35 (IP:Catalog No:116784, 4ug; Detection:Catalog No:116784 1:600) with mouse kidney tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:116784(VPS35 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
VPS35 protein belongs to a group of vacuolar protein sorting (VPS) proteins, which ensure the proper delivery of organelle-specific proteins in eukaryotic cells. VPS35 is the core of a multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Vps35 serves as the core of the multimeric complex by binding directly to Vps26 and Vps29 and SNX1. Northern blot analyses in 16 tissues showed that one transcript of Vps35 with a size of 3.6 kb was highly expressed in brain, heart, testis, ovary, small intestine, spleen, skeletal muscle, and placenta and expressed at moderate or low levels in other tissues. Another transcript of Vps35, a message of 3.0 kb, was also expressed with proportionally lower levels than the 3.6-kb transcript in all the tissues except that the 3.0-kb transcript was not detected in brain. Human Vps35 is mapped at 16q13-q21.
- Malik BR, Godena VK, Whitworth AJ. VPS35 pathogenic mutations confer no dominant toxicity but partial loss of function in Drosophila and genetically interact with parkin. Human molecular genetics. 24(21):6106-17. 2015.
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