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Product Name
UBE2C antibody
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Description
UBE2C Mouse Monoclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 20kD
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
dJ447F3.2 antibody; UBCH10 antibody; UBE2C antibody; Ubiquitin carrier protein C antibody; Ubiquitin protein ligase C antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of UBE2C recombinant protein (Accession Number: NM_007019). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107654(UBE2C Antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells, using UBE2C antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Immunohistochemistry of paraffin-embedded human colon cancer slide using Catalog No:107654(UBE2C Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human colon cancer slide using Catalog No:107654(UBE2C Antibody) at dilution of 1:50
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Background
UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).
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