Canine TNF-alpha/TNFA recombinant protein

Canine TNF-alpha/TNFA recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Tumor necrosis factor alpha

DADB-70P7.1; MGC151434; TNFSF1A; DIF; TNF; TNFSF2; TNFALPHA; TNF-ALPHA; TNFA; TNF-A

P51742

TNF

E. coli

A DNA sequence encoding the soluble form of canine TNF? (NP_001003244.4) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.

Canine

The recombinant canine TNF? consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17 kDa band.

> 96 % as determined by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 0.2-1.2 ng/ml.