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Product Name
Mouse TNF-alpha/TNFA recombinant protein
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Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Protein name
Tumor necrosis factor
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Protein short names
MGC151434; DIF; TNFSF1A; TNF; DADB-70P7.1; TNFALPHA; TNF-ALPHA; TNF-A; TNFA; TNFSF2
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Uniprot ID
Q3U593
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Gene Name
Tnf; Tnfa; Tnfsf2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the soluble form of mouse TNF-? (NP_038721.1) (Leu 80-Leu 235) was expressed, with an initial Met at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse TNF-? consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.04-0.08 ng/mL.
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Validations
Mouse TNF-alpha / TNFA Protein SDS-PAGE
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