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Product Name
STEAP3 antibody
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Description
STEAP3 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in Hela cells. Positive WB detected in HepG2 cells, C6 cells. Positive IP detected in C6 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
dudlin 2 antibody; Dudulin 2 antibody; hpHyde antibody; hTSAP6 antibody; Metalloreductase STEAP3 antibody; pHyde antibody; STEAP family member 3 antibody; STEAP3 antibody; STMP3 antibody; TSAP6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of STEAP3 recombinant protein (Accession Number: XM_006712614). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:115705(STEAP3 antibody) at dilution of 1:2000
Immunofluorescent analysis of Hela cells, using STEAP3 antibody Catalog No:115705 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-STEAP3 (IP:Catalog No:115705, 4ug; Detection:Catalog No:115705 1:700) with C6 cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:115705(STEAP3 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:115705(STEAP3 Antibody) at dilution of 1:50 (under 10x lens)
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Background
STEAP3 represents STEAP family member 3
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References
- Steinberg F, Gallon M, Winfield M. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nature cell biology. 15(5):461-71. 2013.
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