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Product Name
ST6GAL1 antibody
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Description
ST6GAL1 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in U-937 cells, human liver tissue, mouse skeletal muscle tissue, mouse spleen tissue, Raji cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IP, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Alpha 2 antibody; 6 ST 1 antibody; B cell antigen CD75 antibody; CD75 antibody; Sialyltransferase 1 antibody; SIAT1 antibody; ST6Gal I antibody; ST6GAL1 antibody; ST6GalI antibody; ST6N antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ST6GAL1 recombinant protein (Accession Number: XM_017007065). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:115628(ST6GAL1 Antibody) at dilution of 1:600
IP Result of anti-ST6GAL1 (IP:Catalog No:115628, 4ug; Detection:Catalog No:115628 1:600) with Raji cells lysate 2800ug.
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Background
ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa.
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