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Product Name
Anti-ST6GAL1/ST6GAL-I antibody
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Description
Rabbit polyclonal to ST6GAL1/ST6GAL-I
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Tested applications
ELISA, WB, IP
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Species reactivity
Human ST6GAL1 / ST6GAL-I
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Alternative names
ST6N antibody; SIAT1 antibody; ST6GalI antibody; Siat1 antibody; St6gal antibody; St6galI antibody; AW742324 antibody; St6Gal-I antibody; AW742324 antibody; CD75 antibody; MGC116663 antibody; MGC48859 antibody; Siat1 antibody; SIAT1 antibody; St6gal antibody; St6gal1 antibody; ST6GAL1 antibody; St6galI antibody; ST6GalI antibody; ST6N antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human ST6GAL1 / ST6GAL-I (rh ST6GAL1 / ST6GAL-I; P15907; Lys 27-Cys406). ST6GAL1 / ST6GAL-I specific IgG was purified by Human ST6GAL1 / ST6GAL-I affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ST6GAL1. The detection limit for Human ST6GAL1 is approximately 0.00245 ng/well.
IP: 4-6 μg/mg of lysate
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Validations
ST6GAL1 / ST6GAL-I Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
ST6GAL1 / ST6GAL-I Antibody, Rabbit PAb, Antigen Affinity Purified
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Background
Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein which belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the?Golgi?but which can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
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References
- Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206.
- Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68.
- Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414.
- Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"