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  • SF3B1 Polyclonal Antibody

SF3B1 Polyclonal Antibody

Cat.#: 162828

Special Price 174.2 USD

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Product Information

  • Product Name
    SF3B1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to SF3B1
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Alternative names
    SF3B1 antibody; Hsh155 antibody; MDS antibody; PRP10 antibody; PRPF10 antibody; SAP155 antibody; SF3b155 antibody; splicing factor 3B subunit 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - SF3B1 Polyclonal Antibody

    Western blot - SF3B1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using SF3B1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • References
  • Antibodies

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"