Polyclonal antibody to Phospho-SF3B1-T313
Human, Mouse, Rat
SF3B1 antibody; Hsh155 antibody; MDS antibody; PRP10 antibody; PRPF10 antibody; SAP155 antibody; SF3b155 antibody; splicing factor 3B subunit 1 antibody
Antigen: A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-SF3B1-T313 pAb
Western blot analysis of extracts of various cell lines, using Phospho-SF3B1-T313 antibody at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
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