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  • Phospho-SF3B1-T313 pAb

Phospho-SF3B1-T313 pAb

Cat.#: 166540

Special Price 232.3 USD

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Product Information

  • Product Name
    Phospho-SF3B1-T313 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-SF3B1-T313
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    SF3B1 antibody; Hsh155 antibody; MDS antibody; PRP10 antibody; PRPF10 antibody; SAP155 antibody; SF3b155 antibody; splicing factor 3B subunit 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphorylated peptide around T313 of human SF3B1 (NP_036565.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - Phospho-SF3B1-T313 pAb

    Western blot - Phospho-SF3B1-T313 pAb

    Western blot analysis of extracts of various cell lines, using Phospho-SF3B1-T313 antibody at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • References
  • Antibodies

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"