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Product Name
Human PAK3 (His Tag) recombinant protein
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Description
Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.
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Protein name
Serine/threonine-protein kinase PAK 3
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Protein short names
MRX47; STK4; PAK-3; PAK3BETA; HPAK3; BPAK; RP23-45O6.1; PAK65ALPHA; OPHN3; RP5-914P14.3; CDKN1A; MRX30; PAK65BETA
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Uniprot ID
O75914
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Gene Name
PAK3; OPHN3
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human PAK3 consists of 554 amino acids and predicts a molecular mass of 62 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 98 nmol/min/mg using MBP as substrate.
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Validations
Human PAK3 Protein (His Tag) SDS-PAGE
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