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Anti-PAK3 antibody

Cat.#: 176594

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-PAK3 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to PAK3
  • Tested applications
    WB, ICC
  • Species reactivity
    Mouse, Rat, Human
  • Alternative names
    ARA antibody; bPAK antibody; MRX30 antibody; MRX47 antibody; OPHN3 antibody; PAK-3 antibody; XLID30 antibody; PAK3beta antibody; beta-PAK antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human pak3 aa 1-150.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:50-1:100

  • Validations

    Fig1:; Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of PAK3 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2:; ICC staining of PAK3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    Fig2:; ICC staining of PAK3 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody ( 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Background
    O75914
  • References
    • Duarte K. et. al. PAK3 mutations responsible for severe intellectual disability and callosal agenesis inhibit cell migration. Neurobiol Dis. 2020 Mar
    • Iida A. et. al. A novel PAK3 pathogenic variant identified in two siblings from a Japanese family with X-linked intellectual disability: case report and review of the literature. Cold Spring Harb Mol Case Stud. 2019 Dec

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"