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Product Name
RND3 antibody
- Documents
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Description
RND3 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human pancreas tissue. Positive WB detected in mouse skeletal muscle tissue, HeLa cells, HepG2 cells, human liver tissue, human placenta tissue, human spleen tissue. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARHE antibody; memB antibody; Protein MemB antibody; Rho family GTPase 3 antibody; Rho8 antibody; RhoE antibody; RND3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RND3 recombinant protein (Accession Number: NM_005168). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:114734(RND3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114734(RND3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:114734(RND3 antibody) at dilution of 1:50 (under 40x lens)
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Background
Rnd3, also known as RhoE, belongs to the Rnd subclass of the Rho family of small guanosine triphosphate (GTP)-binding proteins. Rnd proteins are unique due to their inability to switch from a GTP-bound to GDP-bound conformation. The basic role of Rnd3 is to report as an endogenous antagonist of RhoA signaling-mediated actin cytoskeleton dynamics, which specifically contributes to cell migration and neuron polarity. Rnd3 also plays a critical role in arresting cell cycle distribution, inhibiting cell growth, and inducing apoptosis and differentiation.
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References
- Xia W, Li J, Chen L. MicroRNA-200b regulates cyclin D1 expression and promotes S-phase entry by targeting RND3 in HeLa cells. Molecular and cellular biochemistry. 344(1-2):261-6. 2010.
- Zhou Y, Li S, Huang Q, Xie L, Zhu X. Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3. Journal of molecular cell biology. 5(4):239-49. 2013.
- Zhu Z, Todorova K, Lee KK. Small GTPase RhoE/Rnd3 is a critical regulator of Notch1 signaling. Cancer research. 74(7):2082-93. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"