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Product Name
PLDN antibody
- Documents
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Description
PLDN Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, HeLa cells, human brain tissue, human heart tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PALLID antibody; Pallid protein homolog antibody; Pallidin antibody; pallidin homolog (mouse) antibody; PLDN antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PLDN recombinant protein (Accession Number: NM_012388). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113952(PLDN antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113952(PLDN antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-PLDN (IP:Catalog No:113952, 3ug; Detection:Catalog No:113952 1:500) with K-562 cells lysate 3600ug.
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Background
Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion. Pallidin is a 25 kDa subunit of the octamer BLoc-1.
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References
- Salazar G, Zlatic S, Craige B, Peden AA, Pohl J, Faundez V. Hermansky-Pudlak syndrome protein complexes associate with phosphatidylinositol 4-kinase type II alpha in neuronal and non-neuronal cells. The Journal of biological chemistry. 284(3):1790-802. 2009.
- Gokhale A, Larimore J, Werner E. Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(11):3697-711. 2012.
- Delevoye C, Heiligenstein X, Ripoll L. BLOC-1 Brings Together the Actin and Microtubule Cytoskeletons to Generate Recycling Endosomes. Current biology : CB. 26(1):1-13. 2016.
- Han MH, Hu Z, Chen CY. Dysbindin-associated proteome in the p2 synaptosome fraction of mouse brain. Journal of proteome research. 13(11):4567-80. 2014.
- Gokhale A, Mullin AP, Zlatic SA. The N-ethylmaleimide-sensitive factor and dysbindin interact to modulate synaptic plasticity. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(19):7643-53. 2015.
- Pu J, Schindler C, Jia R, Jarnik M, Backlund P, Bonifacino JS. BORC, a multisubunit complex that regulates lysosome positioning. Developmental cell. 33(2):176-88. 2015.
- Zlatic SA, Grossniklaus EJ, Ryder PV. Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. Molecular biology of the cell. 24(15):2378-88. 2013.
- Ryder PV, Vistein R, Gokhale A, Seaman MN, Puthenveedu MA, Faundez V. The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα. Molecular biology of the cell. 24(14):2269-84. 2013.
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