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Product Name
Pikachurin antibody
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Description
Pikachurin Rabbit Polyclonal antibody. Positive IF detected in SH-SY5Y cells. Positive WB detected in human placenta tissue, PC-3 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Agrin like protein antibody; AGRINL antibody; AGRNL antibody; EGFLAM antibody; FLJ39155 antibody; Pikachurin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Pikachurin recombinant protein (Accession Number: NM_182801). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:113905(EGFLAM antibody) at dilution of 1:400
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:113905(EGFLAM Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).
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