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Product Name
Phospho-Estrogen Receptor beta-S105 pAb
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Description
Polyclonal antibody to Phospho-Estrogen Receptor beta-S105
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
ESR2 antibody; ER-BETA antibody; ESR-BETA antibody; ESRB antibody; ESTRB antibody; Erb antibody; NR3A2 antibody; estrogen receptor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S105 of human Estrogen Receptor beta
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - Phospho-Estrogen Receptor beta-S105 pAb
Western blot analysis of extracts of HepG2 cells, using Phospho-Estrogen Receptor beta-S105 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
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Background
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"