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  • PHKB antibody
  • PHKB antibody
  • PHKB antibody
  • PHKB antibody

PHKB antibody

Cat.#: 113820

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Product Information

  • Product Name
    PHKB antibody
  • Documents
  • Description
    PHKB Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, mouse skeletal muscle tissue. Positive IHC detected in human skeletal muscle tissue, human liver tissue. Observed molecular weight by Western-blot: 124 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DKFZp781E15103 antibody; FLJ41698 antibody; PHKB antibody; phosphorylase kinase antibody; beta antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PHKB recombinant protein (Accession Number: BC033657). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113820(PHKB antibody) at dilution of 1:1000

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113820(PHKB antibody) at dilution of 1:1000

    IP Result of anti-PHKB (IP:Catalog No:113820, 4ug; Detection:Catalog No:113820 1:500) with mouse heart tissue lysate 3200ug.

    IP Result of anti-PHKB (IP:Catalog No:113820, 4ug; Detection:Catalog No:113820 1:500) with mouse heart tissue lysate 3200ug.

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113820(PHKB Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113820(PHKB Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113820(PHKB Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using Catalog No:113820(PHKB Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.
  • References
    • Lai G, Wu J, Liu X, Zhao Y. 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway. Molecular endocrinology (Baltimore, Md.). 26(11):1907-16. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"