Human OXSR1/OSR1 (GST Tag) recombinant protein

Human OXSR1/OSR1 (GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Oxidative stress-responsive 1 protein (OXSR1), also known as Serine/threonine-protein kinase OSR1, is a member of the Ser/Thr protein kinase family of proteins. OXSR1 regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. OXSR1 is a 58 kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines. The amino acid (aa) sequence of the predicted OXSR1 protein is 39% identical to that of human SOK1. Of potential regulators surveyed, endogenous OXSR1 is activated only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 did not increase the activity of coexpressed JNK, nor did it activate three other MAPKs, p38, ERK2, and ERK5. Phosphorylation by OXSR1 modulates the G protein sensitivity of PAK isoforms. The OXSR1 and SPAK are key enzymes in a signalling cascade regulating the activity of Na+/K+/2Cl- co-transporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which recognizes a unique peptide (Arg-Phe-Xaa-Val) motif. The OXSR1 and SPAK kinases specifically recognize their upstream activators and downstream substrates.

Oxidative-stress responsive 1, isoform CRA_a

KIAA1101; AI462649; 2210022N24RIK; MKIAA1101; 2810422B09RIK; AW209236; OXSR1; OSR1

O95747

OXSR1; hCG_42429

Baculovirus-Insect Cells

A DNA sequence encoding the full length of human OXSR1 (NP_005100.1) (Met 1-Ser 527) was fused with the GST tag at the N-terminus.

Human

The recombinant human GST/OXSR1 chimera consists of 752 amino acids and predicts a molecular mass of 84 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.

> 88 % as determined by SDS-PAGE

Kinase activity untested