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Product Name
OXSR1 antibody
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Description
OXSR1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human liver tissue, human testis tissue. Positive IHC detected in human prostate cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1101 antibody; OSR1 antibody; oxidative stress responsive 1 antibody; OXSR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OXSR1 recombinant protein (Accession Number: NM_005109). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113529(OXSR1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113529(OXSR1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113529(OXSR1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using OXSR1 antibody Catalog No:113529 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-OXSR1 (IP:Catalog No:113529, 4ug; Detection:Catalog No:113529 1:500) with HeLa cells lysate 3200ug.
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Background
Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).
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