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  • NPHP3 antibody
  • NPHP3 antibody
  • NPHP3 antibody
  • NPHP3 antibody
  • NPHP3 antibody

NPHP3 antibody

Cat.#: 113213

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Special Price 239.0 USD

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Product Information

  • Product Name
    NPHP3 antibody
  • Documents
  • Description
    NPHP3 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in hTERT-RPE1 cells. Positive IP detected in mouse testis tissue. Positive WB detected in human testis tissue, HEK-293 cells, HepG2 cells, mouse liver tissue, mouse testis tissue. Observed molecular weight by Western-blot: 145-151 kDa
  • Tested applications
    ELISA, WB, IF, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DKFZp667K242 antibody; DKFZp781K1312 antibody; FLJ30691 antibody; FLJ36696 antibody; KIAA2000 antibody; MKS7 antibody; NephrOCystin 3 antibody; NPHP3 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NPHP3 recombinant protein (Accession Number: BC068082). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    IF result (the base and the lower half of cilia stain) of anti-NPHP3 (Catalog No:113213, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

    IF result (the base and the lower half of cilia stain) of anti-NPHP3 (Catalog No:113213, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

    human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113213(NPHP3 antibody) at dilution of 1:500

    human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113213(NPHP3 antibody) at dilution of 1:500

    IP Result of anti-NPHP3 (IP:Catalog No:113213, 4ug; Detection:Catalog No:113213 1:1000) with mouse testis tissue lysate 4000ug.

    IP Result of anti-NPHP3 (IP:Catalog No:113213, 4ug; Detection:Catalog No:113213 1:1000) with mouse testis tissue lysate 4000ug.

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113213(NPHP3 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    NPHP3 represents nephronophthisis 3 (adolescent)
  • References
    • Su S, Phua SC, DeRose R. Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. Nature methods. 10(11):1105-7. 2013.
    • Schwarz N, Carr AJ, Lane A. Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelium cells. Human molecular genetics. 24(4):972-86. 2015.
  • Recombinant Proteins
  • cDNA Clones

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"