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Product Name
NLRC4 Polyclonal Antibody
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Description
Polyclonal antibody to NLRC4
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
NLRC4 antibody; AIFEC antibody; CARD12 antibody; CLAN antibody; CLAN1 antibody; CLANA antibody; CLANB antibody; CLANC antibody; CLAND antibody; CLR2.1 antibody; FCAS4 antibody; IPAF antibody; NLR family CARD domain containing 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-159 of mouse NLRC4 (NP_001028539.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NLRC4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - NLRC4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung using NLRC4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NLRC4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using NLRC4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NLRC4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using NLRC4 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NLRC4 Polyclonal Antibody
Immunofluorescence analysis of mouse lung using NLRC4 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - NLRC4 Polyclonal Antibody
Immunofluorescence analysis of THP-1 cells using NLRC4 antibody . Blue: DAPI for nuclear staining.
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Background
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"