NF200 Mouse Monoclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Positive WB detected in pig brain tissue. Observed molecular weight by Western-blot: 200 kDa
ELISA, WB, IF, IHC
Human, Pig; other species not tested.
This antibody was obtained by immunization of NF200 recombinant protein (Accession Number: BC014185). Purification method: Protein A purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
pig brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107331(NF200 Antibody) at dilution of 1:4000
Immunohistochemical of paraffin-embedded human brain using Catalog No:107331(NF200 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:107331(NF200 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:107331(NF200 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.
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