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Product Name
MTERFD1 Polyclonal Antibody
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Description
Polyclonal antibody to MTERFD1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
MTERF3 antibody; CGI-12 antibody; MTERFD1 antibody; mitochondrial transcription termination factor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 69-300 of human MTERFD1 (NP_057026.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - MTERFD1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MTERFD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - MTERFD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using MTERFD1 antibody at dilution of 1:100 (40x lens).
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Background
Binds promoter DNA and regulates initiation of transcription. Required for normal mitochondrial transcription and translation, and for normal assembly of mitochondrial respiratory complexes. Required for normal mitochondrial function (By similarity). Maintains 16S rRNA levels and functions in mitochondrial ribosome assembly by regulating the biogenesis of the 39S ribosomal subunit (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"