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Product Name
MonoMethyl-Histone H4-R3 pAb
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Description
Polyclonal antibody to MonoMethyl-Histone H4-R3
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat, Other (Wide Range)
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Alternative names
HIST2H4A antibody; FO108 antibody; H4 antibody; H4/n antibody; H4F2 antibody; H4FN antibody; HIST2H4 antibody; histone H4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human MonoMethyl-Histone H4-R3
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200 -
Validations
Western blot - MonoMethyl-Histone H4-R3 pAb
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-R3 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - MonoMethyl-Histone H4-R3 pAb
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 antibody . Blue: DAPI for nuclear staining.
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Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"