MCM5 Polyclonal Antibody

Cat.#: 164553

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Product Information

  • Product Name
    MCM5 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MCM5
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    MCM5 antibody; CDC46 antibody; P1-CDC46 antibody; DNA replication licensing factor MCM5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:1000 - 1:3000
    IF 1:50 - 1:200
  • Validations

    Western blot - MCM5 Polyclonal Antibody

    Western blot - MCM5 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MCM5 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunofluorescence - MCM5 Polyclonal Antibody

    Immunofluorescence - MCM5 Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using MCM5 antibody . Blue: DAPI for nuclear staining.

  • Background
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"