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Product Name
MCM5 Polyclonal Antibody
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Description
Polyclonal antibody to MCM5
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MCM5 antibody; CDC46 antibody; P1-CDC46 antibody; DNA replication licensing factor MCM5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MCM5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MCM5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - MCM5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using MCM5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCM5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using MCM5 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MCM5 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using MCM5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Interacts with MCMBP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"