-
Product Name
LAMP3 antibody
- Documents
-
Description
LAMP3 Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue, human placenta tissue, human testis tissue. Positive WB detected in mouse lung tissue, A375 cells, A549 cells, mouse thymus tissue, rat lung tissue. Positive IP detected in A549 cells. Observed molecular weight by Western-blot: 70 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
CD208 antibody; DC LAMP antibody; DCLAMP antibody; LAMP antibody; LAMP 3 antibody; LAMP3 antibody; Protein TSC403 antibody; TSC403 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of LAMP3 recombinant protein (Accession Number: NM_014398). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:112278(LAMP3 antibody) at dilution of 1:1000
IP Result of anti-LAMP3 (IP:Catalog No:112278, 4ug; Detection:Catalog No:112278 1:500) with A549 cells lysate 2800ug.
Immunohistochemical of paraffin-embedded human lung using Catalog No:112278(LAMP3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:112278(LAMP3 antibody) at dilution of 1:50 (under 40x lens)
-
Background
LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).
-
References
- Lonskaya I, Shekoyan AR, Hebron ML, Desforges N, Algarzae NK, Moussa CE. Diminished parkin solubility and co-localization with intraneuronal amyloid-β are associated with autophagic defects in Alzheimer's disease. Journal of Alzheimer's disease : JAD. 33(1):231-47. 2013.
- Yeganeh B, Ghavami S, Kroeker AL. Suppression of influenza A virus replication in human lung epithelial cells by noncytotoxic concentrations bafilomycin A1. American journal of physiology. Lung cellular and molecular physiology. 308(3):L270-86. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"