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Product Name
KGA-Specific antibody
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Description
KGA-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human brain tissue, human heart tissue, K-562 cells, mouse brain tissue, mouse kidney tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human kidney tissue, human brain tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AAD20 antibody; DKFZp686O15119 antibody; FLJ10358 antibody; GLS antibody; GLS1 antibody; glutaminase antibody; K glutaminase antibody; KGA antibody; KGA-specific antibody; KIAA0838 antibody; L glutamine amidohydrolase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KGA-Specific recombinant protein (Accession Number: NM_014905). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112035(GLS antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112035(GLS antibody) at dilution of 1:100 (under 40x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112035(GLS Antibody) at dilution of 1:600
IP Result of anti-GLS (IP:Catalog No:112035, 3ug; Detection:Catalog No:112035 1:800) with HEK-293 cells lysate 3200ug.
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Background
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA,GAM,GAC. This antibody is specific to KGA.
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References
- Jacque N, Ronchetti AM, Larrue C. Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. Blood. 126(11):1346-56. 2015.
- Herranz D, Ambesi-Impiombato A, Sudderth J. Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia. Nature medicine. 21(10):1182-9. 2015.
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