Human gp130/CD130 (His & Fc Tag) recombinant protein

Human gp130/CD130 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.

Interleukin-6 receptor subunit beta

GP130-RAPS; CDW130; IL6ST; IL-6RB; BB405851; IL6R-BETA; D13ERTD699E; 5133400A03RIK; AA389424; GP130; CD130

Q17RA0

IL6ST

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ile 618) of human IL6ST (NP_002175.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human IL6ST/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 844 amino acids and has a predicted molecular mass of 96 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL6ST/Fc monomer is approximately 125-140 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind human IL11Ra in a functional ELISA.
2. Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells (Saito, T. et al. 1991, J. Immunol. 147:168.). The ED₅₀ for this effect is typically 0.05-0.15 μg/ml in the presence of 50 ng/ml recombinant human IL6sR and 100 ng/ml recombinant human IL6.