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  • IFIT3 Polyclonal Antibody
  • IFIT3 Polyclonal Antibody
  • IFIT3 Polyclonal Antibody
  • IFIT3 Polyclonal Antibody

IFIT3 Polyclonal Antibody

Cat.#: 164238

Special Price 174.2 USD

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Product Information

  • Product Name
    IFIT3 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to IFIT3
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    IFIT3 antibody; CIG-49 antibody; GARG-49 antibody; IFI60 antibody; IFIT4 antibody; IRG2 antibody; ISG60 antibody; P60 antibody; RIG-G antibody; cig41 antibody; interferon-induced protein with tetratricopeptide repeats 3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:1000
    IHC 1:100 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - IFIT3 Polyclonal Antibody

    Western blot - IFIT3 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using IFIT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.

    Immunohistochemistry - IFIT3 Polyclonal Antibody

    Immunohistochemistry - IFIT3 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - IFIT3 Polyclonal Antibody

    Immunohistochemistry - IFIT3 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - IFIT3 Polyclonal Antibody

    Immunofluorescence - IFIT3 Polyclonal Antibody

    Immunofluorescence analysis of U2OS cells using IFIT3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"