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Product Name
IFIT3 Polyclonal Antibody
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Description
Polyclonal antibody to IFIT3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
IFIT3 antibody; CIG-49 antibody; GARG-49 antibody; IFI60 antibody; IFIT4 antibody; IRG2 antibody; ISG60 antibody; P60 antibody; RIG-G antibody; cig41 antibody; interferon-induced protein with tetratricopeptide repeats 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - IFIT3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IFIT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry - IFIT3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using IFIT3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - IFIT3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using IFIT3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - IFIT3 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using IFIT3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"