Human VCAM-1/CD106 (His Tag) recombinant protein

Cat.#: 503378

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Product Information

  • Product Name
    Human VCAM-1/CD106 (His Tag) recombinant protein
  • Documents
  • Description
    Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.
  • Protein name
    Vascular cell adhesion protein 1
  • Protein short names
    DKFZP779G2333; MGC99561; VCAM-1; INCAM-100; CD106; VCAM1
  • Uniprot ID
    P19320
  • Gene Name
    VCAM1; L1CAM
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Phe 25-Pro 697) of human VCAM1 (NP_001069.1) was fused with a polyhistidine tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human VCAM1 consists of 689 amino acids and predicts a molecular mass of 76 kDa. As a result of glycosylation, rhVCAM1 migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (5 μg/mL, 100 μL/well) approximately 60% will adhere for 1 hour incubation at 37℃.
  • Validations

    Human VCAM-1 / CD106 Protein (His Tag) SDS-PAGE

    Human VCAM-1 / CD106 Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"