NovoPro
Cart 2
  • Human SHH / Sonic hedgehog (aa 1-197, His Tag) recombinant protein

Human SHH / Sonic hedgehog (aa 1-197, His Tag) recombinant protein

Cat.#: 504207

Special Price 77.1 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Human SHH / Sonic hedgehog (aa 1-197, His Tag) recombinant protein
  • Documents
  • Description
    Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.
  • Protein name
    Sonic hedgehog protein
  • Protein short names
    HLP3; HHG1; HX; SONIC HEDGEHOG HOMOLOG; TPT; HXL3; HPE3; HEMIMELIC EXTRA TOES; M100081; DSH; MCOPCB5; SMMCI; SONIC HEDGEHOG PROTEIN; TPTPS; 9530036O11RIK; SHORT DIGITS; HHG-1
  • Uniprot ID
    Q15465
  • Gene Name
    SHH
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465), that is Sonic hedgehog protein N-product, was fused with a polyhistidine tag at the C-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.
    The ED50 for this effect is typically 2-10 μg/mL.
  • Validations

    Human SHH / Sonic hedgehog Protein (aa 1-197, His Tag) SDS-PAGE

    Human SHH / Sonic hedgehog Protein (aa 1-197, His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"