Human K-Ras (His Tag) recombinant protein

Cat.#: 504300

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Product Information

  • Product Name
    Human K-Ras (His Tag) recombinant protein
  • Documents
  • Description
    K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example RasGAP. In turn K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.
  • Protein name
    GTPase KRas
  • Protein short names
    NS3; K-RAS2B; RAS; K-RAS4A; K-RAS; RASK2; C-K-RAS; AI929937; RP24-359O2.1; CFC2; KI-RAS; KRAS-2; KRAS; KRAS2; KRAS1; P21B; NS; K-RAS4B; K-RAS2A; MGC7141
  • Uniprot ID
    P01116
  • Gene Name
    KRAS; KRAS2; RASK2
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the native human KRAS (AAH13572.1) (Thr 2-Cys 185) was expressed, with a polyhistide tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human KRAS consisting of 195 amino acids and has a calculated molecular mass of 22.5 kDa. It migrates as an 25 kDa band in SDS-PAGE under reducing conditions as predicted
  • Purity
    > 90 % as determined by SDS-PAGE
  • Validations

    Human K-Ras / K-Ras Protein (His Tag) SDS-PAGE

    Human K-Ras / K-Ras Protein (His Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"