-
Product Name
Human ECE2/ECE-2 (Fc Tag) recombinant protein
- Documents
-
Description
Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.
-
Protein name
Endothelin-converting enzyme 2
-
Protein short names
MGC17664; KIAA0604; MGC2408; 9630025D12RIK; BB127715; ECE2; MGC144386; ECE-2; 1810009K13RIK; MGC144385; MGC78487; 6330509A19RIK; HCG_2022032
-
Uniprot ID
O60344
-
Gene Name
ECE2; KIAA0604; UNQ403/PRO740
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the ectodomain of human endothelin converting enzyme 2 isoform A (NP_055508.3) (Gly 199-Trp 883) was fused with the Fc region of human IgG1 at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human Fc/ECE2 is a disulfide-linked homodimeric protein. The reduced monomer consists of 921 amino acids and predicts a molecular mass of 105 kDa. As a result of glycosylation, the apparent molecular mass of rh ECE2/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
-
Purity
> 95 % as determined by SDS-PAGE
-
Validations
Human ECE-2 Protein (Fc Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"