Human CD27 (His Tag) recombinant protein

Human CD27 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

CD27 antigen

CD27; TNFRSF7; TP55; T14; MGC20393; S152.LPFS2; S152

P26842

CD27; TNFRSF7

Baculovirus-Insect Cells

A DNA sequence encoding the human CD27(P26842) ( Met 1-Ile192) was expressed with a C-terminal polyhistidine tag.

Human

The secreted recombinant human CD27 consists of 183 amino acids and predicts a molecular mass of 20.7 KDa. The apparent molecular mass of the protein is approximately 27 KDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human CD27-his (Cat:503763) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (Cat:502332), The EC₅₀ of biotinylated human CD70-Fc (Cat:502332) is 57-85 ng/mL.