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Product Name
HE4 antibody
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Description
HE4 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human testis tissue. Positive WB detected in Transfected HEK-293 cells.
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
dJ461P17.6 antibody; HE4 antibody; WAP5 antibody; WFDC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HE4 recombinant protein (Accession Number: NM_006103). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
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Validations
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111391(HE4 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:111391(HE4 Antibody) at dilution of 1:100 (under 10x lens).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:111391(HE4 Antibody) at dilution of 1:100 (under 40x lens).
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Background
WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.
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