HBEGF Polyclonal Antibody
Polyclonal antibody to HBEGF
WB, IHC, IF
Human, Mouse, Rat
HBEGF antibody; DTR antibody; DTS antibody; DTSF antibody; HEGFL antibody; heparin binding EGF like growth factor antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human HBEGF (NP_001936.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot - HBEGF Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HBEGF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Related Products / Services
- Recombinant Proteins
- cDNA Clones
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"