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Product Name
Fibronectin antibody
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Description
Fibronectin Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Positive IF detected in NIH/3T3 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 250 kDa,70 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CIG antibody; Cold insoluble globulin antibody; DKFZp686F10164 antibody; DKFZp686H0342 antibody; DKFZp686I1370 antibody; DKFZp686O13149 antibody; ED B antibody; Fibronectin antibody; fibronectin 1 antibody; FINC antibody; FN1 antibody; FNZ antibody; GFND antibody; GFND2 antibody; LETS antibody; MSF antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Fibronectin recombinant protein (Accession Number: BC005858). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110659(FN1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110659(FN1 antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110659(FN1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-FN1 (IP:Catalog No:110659, 4ug; Detection:Catalog No:110659 1:300) with NIH/3T3 cells lysate 4000ug.
Immunofluorescent analysis of NIH/3T3 cells using Catalog No:110659(FN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)
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References
- Zhang X, Xu J, Wang J. Reduction of microRNA-206 contributes to the development of bronchopulmonary dysplasia through up-regulation of fibronectin 1. PloS one. 8(9):e74750. 2013.
- Guo Z, Wu J, Zhao J. IL-33 promotes airway remodeling and is a marker of asthma disease severity. The Journal of asthma : official journal of the Association for the Care of Asthma. 51(8):863-9. 2014.
- Song Q, Xu Y, Yang C. miR-483-5p promotes invasion and metastasis of lung adenocarcinoma by targeting RhoGDI1 and ALCAM. Cancer research. 74(11):3031-42. 2014.
- Miao L, Xiong X, Lin Y. miR-203 inhibits tumor cell migration and invasion via caveolin-1 in pancreatic cancer cells. Oncology letters. 7(3):658-662. 2014.
- Huang C, Wang H, Pan J. Benzalkonium chloride induces subconjunctival fibrosis through the COX-2-modulated activation of a TGF-β1/Smad3 signaling pathway. Investigative ophthalmology & visual science. 55(12):8111-22. 2014.
- Dong D, Yin L, Qi Y, Xu L, Peng J. Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats. Nutrients. 7(6):4829-50. 2015.
- Liang C, Li X, Zhang L, Cui D, Quan X, Yang W. The anti-fibrotic effects of microRNA-153 by targeting TGFBR-2 in pulmonary fibrosis. Experimental and molecular pathology. 99(2):279-85. 2015.
- Kusumi K, Smith S, Barr-Beare E. Pediatric Origins of Nephrolithiasis-Associated Atherosclerosis. The Journal of pediatrics. 167(5):1074-80.e2. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"