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Product Name
Fibronectin antibody
- Documents
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Description
Fibronectin Mouse Monoclonal antibody. Positive IF detected in NIH/3T3 cells. Positive WB detected in Human brain, human liver tissue. Observed molecular weight by Western-blot: 262 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CIG antibody; Cold insoluble globulin antibody; DKFZp686F10164 antibody; DKFZp686H0342 antibody; DKFZp686I1370 antibody; DKFZp686O13149 antibody; ED B antibody; Fibronectin antibody; fibronectin 1 antibody; FINC antibody; FN1 antibody; FNZ antibody; GFND antibody; GFND2 antibody; LETS antibody; MSF antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Fibronectin recombinant protein (Accession Number: BC005858). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107214(FN1 antibody) at dilution of 1:500
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:107214(FN1 Antibody) at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"