-
Product Name
FHIT antibody
- Documents
-
Description
FHIT Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in K562 cells, K-562 cells. Observed molecular weight by Western-blot: 17 kDa
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
AP3A hydrolase antibody; AP3Aase antibody; Bis(5 adenosyl) triphosphatase antibody; Dinucleosidetriphosphatase antibody; FHIT antibody; FRA3B antibody; fragile histidine triad gene antibody
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of FHIT recombinant protein (Accession Number: NM_001166243). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107210(FHIT antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107210(FHIT Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107210(FHIT Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using FHIT antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
-
Background
The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"