Human Fragile histidine triad / FHIT (His Tag) recombinant protein

Human Fragile histidine triad / FHIT (His Tag) recombinant protein

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Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

Bis(5'-adenosyl)-triphosphatase

FRAGILE HISTIDINE TRIAD GENE; DINUCLEOSIDETRIPHOSPHATASE; AP3A HYDROLASE; FRAGILE HISTIDINE TRIAD PROTEIN; TUMOR SUPPRESSOR PROTEIN; DIADENOSINE 5'; FRA14A2; DIADENOSINE TRIPHOSPHATE HYDROLASE; FRAGILE HISTIDINE TRIAD; FHIT; BIS(5'-ADENOSYL)-TRIPHOSPHATASE; FRA3B; 5'''-P1; P3-TRIPHOSPHATE HYDROLASE; AP3AASE; AW045638

P49789

FHIT

E. coli

A DNA sequence encoding the human FHIT (P49789) (Met1-Gln147) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human FHIT consists of 153 amino acids and predicts a molecular mass of 17.7 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE