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Product Name
EXO1 Polyclonal Antibody
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Description
Polyclonal antibody to EXO1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
EXO1 antibody; HEX1 antibody; hExoI antibody; exonuclease 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - EXO1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EXO1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - EXO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EXO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using EXO1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EXO1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using EXO1 antibody at dilution of 1:100 (40x lens).
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Background
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
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