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Product Name
EPLIN antibody
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Description
EPLIN Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, human placenta tissue, PC-3 cells. Observed molecular weight by Western-blot: 90kd, 110kd
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EPLIN antibody; LIM domain and actin binding 1 antibody; LIMA1 antibody; SREBP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPLIN recombinant protein (Accession Number: BC001247). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110388(LIMA1 antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using LIMA1 antibody Catalog No:110388 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-LIMA1 (IP:Catalog No:110388, 4ug; Detection:Catalog No:110388 1:500) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:110388(LIMA1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:110388(LIMA1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
LIMA1 represents LIM domain and actin binding 1
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