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Product Name
ENTPD1 antibody
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Description
ENTPD1 Mouse Monoclonal antibody. Positive WB detected in Jurkat cells. Positive IF detected in HeLa cells. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
ATPDase antibody; CD39 antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase 1 antibody; Ecto ATPase 1 antibody; Ecto ATPDase 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; NTPDase 1 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of ENTPD1 recombinant protein (Accession Number: NM_001164183). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107253(ENTPD1 Antibody) at dilution of 1:500
Immunofluorescent analysis of HeLa cells using Catalog No:107253(ENTPD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
1X10^6 Jurkat cells were stained with 0.2ug ENTPD1 antibody (Catalog No:107253, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.
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Background
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) locates to the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.
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